TABLE 2.Electrophysiological properties of four types of EGFP-expressing cells
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Group 1 (n = 17) | Group 2 (n = 7) | Group 3 (n = 15) | Group 4 (n = 7) | |||||
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Groups 1, 2, 3 >> 4; Groups 1, 2 > 3 | ||||||||
AHP shape, mV | 1.6 ± 0.8 | 1.7 ± 1.0 | 0.8 ± 0.7 | –3.6 ± 0.6 | ||||
Group 3 >> Groups 1, 2; Group 3 > Group 4 | ||||||||
Steady-state firing frequency, Hz | 36.2 ± 9.6 | 28.8 ± 9.0 | 56.6 ± 17.5 | 37.2 ± 13.0 | ||||
Group 1 >> Group 4; Group 2 >> Groups 1, 3, 4 | ||||||||
Membrane time constant, ms | 20.5 ± 6.5 | 31.4 ± 7.7 | 15.3 ± 3.4 | 9.8 ± 3.3 | ||||
Group 3 >> Groups 1, 2, 4 | ||||||||
Sag conductance, nS | 1.7 ± 1.2 | 0.51 ± 0.72 | 3.9 ± 1.2 | 0.95 ± 1.0 | ||||
Group 1 >> Groups 2, 3 | ||||||||
sEPSC peak amplitude, pA | –18.1 ± 4.2 | –31.1 ± 7.2 | –25.7 ± 7.3 | –25.1 ± 7.2 | ||||
Group 1 > Groups 2, 4 | ||||||||
sEPSC rise time, ms | 1.9 ± 0.4 | 1.4 ± 0.4 | 1.6 ± 0.3 | 1.4 ± 0.1 | ||||
Group 1 >> Groups 2, 3, 4 | ||||||||
sEPSC decay time, ms | 8.2 ± 1.4 | 5.0 ± 1.4 | 6.3 ± 1.5 | 4.8 ± 1.3 | ||||
Groups not significantly different (P > 0.1) | ||||||||
Input resistance, MΩ | 205 ± 30 | 224 ± 19 | 212 ± 27 | 213 ± 19 | ||||
Groups not significantly different (P > 0.1) | ||||||||
Resting membrane potential, mV | –58.7 ± 3.6 | –59.0 ± 2.7 | –57.2 ± 3.3 | –60.9 ± 3.8 | ||||
Groups not significantly different (P > 0.5) | ||||||||
Series resistance MΩ | 36.2 ± 9.6 | 28.8 ± 9.0 | 56.6 ± 17.5 | 37.2 ± 13.0 | ||||
Groups not significantly different (P > 0.1) | ||||||||
f–I terminal slope Hz/nA | 36.2 ± 9.6 | 28.8 ± 9.0 | 56.6 ± 17.5 | 37.2 ± 13.0 |
Values are means ± SD, n, number of cells; >, significantly greater with P = 0.05; >>, significantly greater with P = 0.01. Statistically significant differences were determined using a Tukey post hoc means analysis after performing ANOVA.
Electrophysiological parameters were measured as described in METHODS.